6FHU

Crystal structure of BAZ2A PHD zinc finger in complex with H3 3-mer peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9282
Spacegroup name	P 43 21 2
Unit cell lengths	73.385, 73.385, 99.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.030 - 2.000
R-factor	0.19514
Rwork	0.193
R-free	0.23488
RMSD bond length	0.011
RMSD bond angle	1.552
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.030	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.097
Number of reflections	19049	1360
<I/σ(I)>	15.5
Completeness [%]	99.9
Redundancy	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	2.2-2.4 M sodium/potassium phosphate