6FHG
Crystal structure of the Ts2631 endolysin from Thermus scotoductus phage with the unique N-terminal moiety responsible for peptidoglycan anchoring
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.579, 56.094, 116.715 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.693 - 1.950 |
R-factor | 0.2055 |
Rwork | 0.203 |
R-free | 0.24690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lba |
RMSD bond length | 0.006 |
RMSD bond angle | 1.057 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.693 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 26266 | |
<I/σ(I)> | 7.72 | |
Completeness [%] | 91.7 | |
Redundancy | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350 and 0.2 mM di-sodium tartrate |