6FHE
Highly active enzymes by automated modular backbone assembly and sequence design
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2017-07-12 |
| Detector | RIGAKU |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 51.020, 51.020, 296.379 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.020 - 1.930 |
| Rwork | 0.242 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w2f |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1-2575_1692: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.020 | 1.970 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 30737 | 2057 |
| <I/σ(I)> | 7.6 | 1 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 6.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 8% PEG 3,350 0.05M Tri-sodium cidrate pH=5.8 |
