6FHA

Death-associated Protein Kinase 1 (DAPK1) catalytic and auto-regulatory domains with S289A and S308A mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	46.993, 75.820, 93.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.943 - 2.300
R-factor	0.2036
Rwork	0.201
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b4l
RMSD bond length	0.009
RMSD bond angle	1.194
Data reduction software	XDS
Data scaling software	Aimless (7.0.021)
Phasing software	PHASER (Phenix 1.9-1692)
Refinement software	PHENIX (1.9-1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.943	1.950
High resolution limit [Å]	1.878	1.880
Rmerge	0.134	0.705
Rmeas	0.150
Rpim	0.067
Number of reflections	25549
<I/σ(I)>	8.9
Completeness [%]	91.4	36.1
Redundancy	4.9	2.9
CC(1/2)		0.018

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292.15	22% PEG 4000, 0.2M ammonium sulfate, 0.1M sodium sulfate