6FG9

Mouse SORCS2 ectodomain (sortilin related VPS10 domain containing receptor 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.99998
Spacegroup name	C 2 2 21
Unit cell lengths	138.815, 329.275, 131.409
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.203 - 4.200
R-factor	0.2514
Rwork	0.249
R-free	0.29070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4SML 1wgo 4aqo
RMSD bond length	0.002
RMSD bond angle	0.586
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.203	4.260
High resolution limit [Å]	4.000	4.000
Rmerge	0.200
Number of reflections	26077	4652
<I/σ(I)>	5	0.8
Completeness [%]	99.8	99.6
Redundancy	6.4	6.6
CC(1/2)	1.000	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	0.1 M Sodium chloride, 0.02 M Tris pH 7.5, 0.1 M Magnesium chloride hexahydrate and 11 % w/v PEG 1500, final pH 6.4