6FG8

Crystal structure of the BIR3 - SERK1 complex from Arabidopsis thaliana.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-06
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.03320
Spacegroup name	P 1 21 1
Unit cell lengths	52.173, 50.757, 77.425
Unit cell angles	90.00, 96.72, 90.00

Refinement procedure

Resolution	40.810 - 1.250
R-factor	0.15308
Rwork	0.151
R-free	0.18394
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lsc 6fg7
RMSD bond length	0.012
RMSD bond angle	1.745
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.810	1.330
High resolution limit [Å]	1.250	1.250
Rmeas	0.058	1.100
Number of reflections	109876	17072
<I/σ(I)>	15.1	1.5
Completeness [%]	98.8	95.5
Redundancy	6.3	5.7
CC(1/2)	1.000	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	19% PEG 3,350, 1 M LiCl, 0.1 M sodium acetate pH 5.5