6FEZ
Ryegrass mottle virus protease domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2017-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.110, 115.990, 57.920 |
| Unit cell angles | 90.00, 105.27, 90.00 |
Refinement procedure
| Resolution | 57.990 - 2.300 |
| R-factor | 0.23229 |
| Rwork | 0.229 |
| R-free | 0.29018 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zyo |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.767 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.990 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.123 | 0.548 |
| Rmeas | 0.149 | 0.662 |
| Rpim | 0.083 | 0.365 |
| Number of reflections | 19160 | 2981 |
| <I/σ(I)> | 5.3 | 2.1 |
| Completeness [%] | 96.0 | 98.69 |
| Redundancy | 3 | 3.1 |
| CC(1/2) | 0.989 | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES 7.5, 22.5% PEG 3350, 0.22M LiSO4, 3% DMSO |
