6FDB
Positively supercharged variant of the computationally designed cage protein O3-33
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 136.865, 136.865, 559.097 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.594 - 3.619 |
| R-factor | 0.2285 |
| Rwork | 0.225 |
| R-free | 0.28770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vcd |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.598 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.840 |
| High resolution limit [Å] | 3.619 | 3.619 |
| Rmerge | 0.308 | 1.892 |
| Number of reflections | 44350 | 6952 |
| <I/σ(I)> | 5.3 | 0.89 |
| Completeness [%] | 99.5 | 97.4 |
| Redundancy | 6 | |
| CC(1/2) | 0.989 | 0.341 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2 M (NH4)2SO4, 5% 2-propanol |
