6FD1

7-FE FERREDOXIN FROM AZOTOBACTER VINELANDII LOW TEMPERATURE, 1.35 A

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-04
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	54.840, 54.840, 92.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9999.000 * - 1.350
R-factor	0.153
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fd1
RMSD bond length	0.020
RMSD bond angle	0.040
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TRIVIAL (CASE)
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	1.380
High resolution limit [Å]	1.350	1.350
Rmerge	0.057 *
Number of reflections	57315
<I/σ(I)>	21.3	1.7
Completeness [%]	96.5	72
Redundancy	3.3	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.8		anaerobic conditions, Martin, A.E., (1990) Proc. Natl. Acad. Sci. USA., 87, 598. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	0.1 (M)
2	1	1	ammonium sulfate	5.1 (M)
3	1	1	protein	1.0 (mM)