6FCS
The X-ray Structure of Lytic Transglycosylase Slt inactive mutant E503Q from Pseudomonas aeruginosa in complex with NAG-NAMpentapeptide-NAG-NAMpentapeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 63 |
| Unit cell lengths | 166.873, 166.873, 54.509 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 144.520 - 2.900 |
| R-factor | 0.19673 |
| Rwork | 0.193 |
| R-free | 0.26884 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ohu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.361 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 144.520 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.092 | |
| Number of reflections | 19575 | |
| <I/σ(I)> | 9.91 | |
| Completeness [%] | 99.9 | |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 80mM Tris pH 8.5, 12% PEG 8000 and 160mM calcium acetate |
