6FCQ
The X-ray Structure of Lytic Transglycosylase Slt inactive mutant E503Q from Pseudomonas aeruginosa in complex with Bulgecin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97953 |
| Spacegroup name | P 63 |
| Unit cell lengths | 165.715, 165.715, 55.884 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 143.510 - 3.100 |
| R-factor | 0.18313 |
| Rwork | 0.181 |
| R-free | 0.21683 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ohu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.261 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 143.510 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rpim | 0.037 | |
| Number of reflections | 15755 | |
| <I/σ(I)> | 16.53 | |
| Completeness [%] | 97.1 | |
| Redundancy | 16.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 80mM Tris pH 8.5, 12% PEG 8000 and 160mM calcium acetate |
