6FCO
Structural and functional characterisation of Frataxin (FXN) like protein from Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96861 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.058, 89.058, 185.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.290 - 2.030 |
| R-factor | 0.22079 |
| Rwork | 0.219 |
| R-free | 0.25956 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.654 |
| Data reduction software | iMOSFLM (7.2.1) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.290 | 2.106 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.079 | |
| Rpim | 0.800 | |
| Number of reflections | 48810 | 48810 |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 1 | |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2.4 M Sodium malonate and 0.1 M BIS-TRIS propane pH 7.0 |
