6FC6

Bik1 CAP-Gly domain with ETF peptide from Bim1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	53.566, 53.566, 62.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.540 - 1.800
R-factor	0.1894
Rwork	0.185
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Bik1 CAP-Gly domain (PDB ID: 6FC5)
RMSD bond length	0.019
RMSD bond angle	1.678
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.550	1.870
High resolution limit [Å]	1.800	1.800
Number of reflections	8808
<I/σ(I)>	13.2
Completeness [%]	100.0
Redundancy	25.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.4	293	0.1 M sodium cacodylate, 1.0 M sodium citrate.