6FC2

Crystal structure of the eIF4E-Eap1p complex from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99996
Spacegroup name	P 21 21 2
Unit cell lengths	87.574, 95.116, 56.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.263 - 1.920
R-factor	0.175
Rwork	0.173
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fbz chain A
RMSD bond length	0.010
RMSD bond angle	0.747
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.263	1.970
High resolution limit [Å]	1.920	1.920
Number of reflections	36515	2408
<I/σ(I)>	17.5	1.6
Completeness [%]	100.0	99.9
Redundancy	13.2	12.7
CC(1/2)		0.542

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Hepes-NaOH (pH 7.5) 30% (w/v) Jeffamine ED-2001