6FBY
Crystal structure of C-terminal modified Tau peptide-hybrid 4.2b with 14-3-3sigma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977930 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.759, 70.230, 127.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.797 - 1.500 |
| R-factor | 0.1425 |
| Rwork | 0.141 |
| R-free | 0.17980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.850 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.18) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.800 | 46.800 | 1.530 |
| High resolution limit [Å] | 1.500 | 8.220 | 1.500 |
| Rmerge | 0.100 | 0.039 | 1.189 |
| Rmeas | 0.104 | 0.040 | 1.240 |
| Rpim | 0.030 | 0.011 | 0.350 |
| Total number of observations | 1117687 | 8190 | 55607 |
| Number of reflections | 91278 | 664 | 4471 |
| <I/σ(I)> | 15.2 | 51.3 | 2.3 |
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 12.2 | 12.3 | 12.4 |
| CC(1/2) | 0.999 | 0.999 | 0.677 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 277 | 24% PEG400, 10 mM HEPES pH 7.1, 5% glycerol, 0.19 M CaCl 2 |
