6FBM
Crystal structure of GNIP1Aa from Chromobacterium piscinae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION NOVA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-27 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.676, 143.187, 209.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.988 - 2.500 |
| Rwork | 0.230 |
| R-free | 0.27140 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.550 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK |
| Refinement software | REFMAC (5.8.0158 2016/10/03) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmeas | 0.129 | 0.429 |
| Number of reflections | 79981 | 12826 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 96.6 | 97.7 |
| Redundancy | 3.72 | 3.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM citrate, pH 5.6, 2 % Tacsimate, pH 5.0, 14 % (w/v) PEG 3350 |
