6FBJ
monoclonal antibody targeting Matrix metalloproteinase 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-12-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.443, 88.443, 119.341 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.20899 |
| Rwork | 0.207 |
| R-free | 0.24927 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ipu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.579 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0171) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.085 | 0.580 |
| Number of reflections | 24579 | 1203 |
| <I/σ(I)> | 33 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 0.1M CaCl2 0.05M HEPES pH=7 10% PEG 6000 0.005M Betaine HCl |
