6FBG

KlenTaq DNA polymerase processing a modified primer - bearing the modification upstream at the fifth primer nucleotide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00000
Spacegroup name	P 31 2 1
Unit cell lengths	109.288, 109.288, 90.723
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.323 - 1.702
R-factor	0.1962
Rwork	0.195
R-free	0.21700
RMSD bond length	0.004
RMSD bond angle	0.583
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12rc1_2815: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.323	1.711
High resolution limit [Å]	1.702	1.702
Rmeas	0.094	3.071
Number of reflections	132688	21384
<I/σ(I)>	11.39	0.58
Completeness [%]	99.9	99.5
Redundancy	5.17	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	15 % PEG 4000, 0.1 M HEPES, 20 mM manganese(II) chloride, 0.1 M sodium acetate