6FBB

Crystal structure of 14-3-3 sigma in complex with wild-type Shroom3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	82.528, 112.241, 62.725
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.626 - 1.300
R-factor	0.1547
Rwork	0.154
R-free	0.16640
RMSD bond length	0.008
RMSD bond angle	0.894
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.630	1.320
High resolution limit [Å]	1.300	1.300
Number of reflections	71785
<I/σ(I)>	15.9
Completeness [%]	100.0
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.095 M HEPES pH7.3 0.19 M CaCl2 26% PEG400 5% glycerol