6FAT
The crystal structure of a feruloyl esterase C from Fusarium oxysporum.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-18 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.2395 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.536, 87.467, 106.565 |
| Unit cell angles | 90.00, 106.18, 90.00 |
Refinement procedure
| Resolution | 102.350 - 2.300 |
| R-factor | 0.20842 |
| Rwork | 0.207 |
| R-free | 0.23486 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wmt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.072 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.350 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.096 | |
| Number of reflections | 52149 | |
| <I/σ(I)> | 0.095 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289.15 | 30% (v/v) PEG400, 0.1M Tris-HCl pH 8.5 buffer |
