6FAO
Discovery and characterization of a thermostable GH6 endoglucanase from a compost metagenome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.741, 78.683, 85.531 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.910 - 1.880 |
| R-factor | 0.16191 |
| Rwork | 0.158 |
| R-free | 0.20440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2boe |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.738 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.910 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rpim | 0.064 | 0.534 |
| Number of reflections | 27338 | 1636 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.3 | 94.4 |
| Redundancy | 4.9 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1 M (NH4)2SO4, 0.1 M BisTris pH 5.5, 1 % PEG 3350 |
