6FAN
Crystal structure of putative CooT from Carboxydothermus hydrogenoformans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2017-05-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.261, 61.350, 81.118 |
| Unit cell angles | 90.00, 100.41, 90.00 |
Refinement procedure
| Resolution | 79.780 - 2.000 |
| R-factor | 0.23658 |
| Rwork | 0.235 |
| R-free | 0.27517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.096 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.850 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 97738 | |
| <I/σ(I)> | 13.41 | |
| Completeness [%] | 97.6 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 47% (v/v) 2-methylpentane-2,4-diol, 2% (v/v) 2-methyl-2-propanol |
