6FAC

CRYSTAL STRUCTURE OF THE DEAH-BOX HELICASE PRP2 IN COMPLEX WITH ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	122.740, 67.320, 95.620
Unit cell angles	90.00, 106.46, 90.00

Refinement procedure

Resolution	46.410 - 2.050
R-factor	0.2174
Rwork	0.216
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6faa
RMSD bond length	0.010
RMSD bond angle	1.476
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.410	50.000	2.150
High resolution limit [Å]	2.050	50.000	2.050
Rmerge	0.039		0.794
Rmeas	0.045		0.909
Number of reflections	46873		6177
<I/σ(I)>	20.31		2.19
Completeness [%]	99.5		99.6
Redundancy	4.08
CC(1/2)	0.999		0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.06 M MgCl2/CaCl2, 0.1 M HEPES/MOPS pH 7.5, 20% (w/v) Etylene glycol, 10% (w/v) PEG 8000