6FA5

CRYSTAL STRUCTURE OF THE DEAH-BOX HELICASE PRP2 IN COMPLEX WITH ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-31
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.87313
Spacegroup name	P 21 21 21
Unit cell lengths	48.206, 116.412, 118.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.638 - 2.303
R-factor	0.2003
Rwork	0.198
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6faa
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.638	44.638	2.440
High resolution limit [Å]	2.300	6.840	2.300
Rmerge	0.079	0.024	0.853
Rmeas	0.090	0.028	0.968
Total number of observations	128483
Number of reflections	30007	1260	4702
<I/σ(I)>	13.78	42.8	1.91
Completeness [%]	99.1	98.4	97.9
Redundancy	4.282	3.786	4.353
CC(1/2)	0.998	0.999	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	100mM Na-HEPES, 250mM NaCl, 25% PEG6000