6F93

Helicobacter pylori serine hydroxymethyl transferase in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	57.349, 87.548, 162.476
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.851 - 2.800
R-factor	0.1963
Rwork	0.194
R-free	0.23560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dkj
RMSD bond length	0.005
RMSD bond angle	0.795
Data reduction software	Adxv
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.851	2.950
High resolution limit [Å]	2.800	2.800
Rpim	0.113	0.525
Number of reflections	20625	2920
<I/σ(I)>	10.2	2.3
Completeness [%]	99.1	98.7
Redundancy	3.6	3.6
CC(1/2)	0.999	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	20% PEG 3350, 0.2 M Sodium Acetate