6F8Z
Structure of the family GH92 alpha-mannosidase BT3130 from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 272.301, 272.301, 190.047 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.670 - 2.500 |
| R-factor | 0.1826 |
| Rwork | 0.181 |
| R-free | 0.22176 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wvy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.318 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.670 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.210 | 2.000 |
| Rmeas | 0.218 | 2.090 |
| Rpim | 0.060 | 0.579 |
| Number of reflections | 142171 | 6722 |
| <I/σ(I)> | 18.6 | 2.2 |
| Completeness [%] | 99.8 | 96.7 |
| Redundancy | 25 | 24.5 |
| CC(1/2) | 0.998 | 0.735 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 292 | 18% (w/v) PEG 3350, 0.1 M Bis-Tris propane, pH 6.4, 0.2 M NaBr |
