6F8Y
Crystal structure of P. abyssi Sua5 complexed with L-threonine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.230, 142.380, 103.860 |
| Unit cell angles | 90.00, 101.80, 90.00 |
Refinement procedure
| Resolution | 48.780 - 2.860 |
| R-factor | 0.1912 |
| Rwork | 0.188 |
| R-free | 0.25170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eqa |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.671 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.781 | 3.030 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Rmeas | 0.090 | |
| Number of reflections | 49934 | |
| <I/σ(I)> | 10.66 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | ammonium sulfate |
