6F8Y

Crystal structure of P. abyssi Sua5 complexed with L-threonine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98011
Spacegroup name	C 1 2 1
Unit cell lengths	152.230, 142.380, 103.860
Unit cell angles	90.00, 101.80, 90.00

Refinement procedure

Resolution	48.780 - 2.860
R-factor	0.1912
Rwork	0.188
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2eqa
RMSD bond length	0.013
RMSD bond angle	1.671
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.781	3.030
High resolution limit [Å]	2.860	2.860
Rmeas	0.090
Number of reflections	49934
<I/σ(I)>	10.66
Completeness [%]	99.3
Redundancy	3.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	ammonium sulfate