6F87
Crystal structure of P. abyssi Sua5 complexed with L-threonine and PPi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980110 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.440, 144.070, 103.660 |
| Unit cell angles | 90.00, 103.06, 90.00 |
Refinement procedure
| Resolution | 49.030 - 2.620 |
| R-factor | 0.1781 |
| Rwork | 0.175 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eqa |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.783 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.030 | 2.780 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmeas | 0.071 | 0.690 |
| Number of reflections | 64452 | 10212 |
| <I/σ(I)> | 14.14 | 2.11 |
| Completeness [%] | 99.4 | 97.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | ammonium sulfate |
