6F7V
Crystal structure of LkcE E64Q mutant in complex with LC-KA05
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-29 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95371 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 124.834, 124.834, 157.392 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 3.030 |
| R-factor | 0.1938 |
| Rwork | 0.191 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.605 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.900 | 48.900 | 3.110 |
| High resolution limit [Å] | 3.030 | 13.550 | 3.030 |
| Rmerge | 0.111 | 0.035 | 0.760 |
| Rmeas | 0.113 | 0.036 | 0.774 |
| Rpim | 0.021 | 0.007 | 0.142 |
| Total number of observations | 737003 | ||
| Number of reflections | 24814 | 340 | 1757 |
| <I/σ(I)> | 28 | ||
| Completeness [%] | 99.8 | 98.2 | 97.2 |
| Redundancy | 29.7 | 22.5 | 27.9 |
| CC(1/2) | 1.000 | 1.000 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 16 % PEG 8000, 160 mM calcium acetate, Bis Tris 100 mM pH 6.5 |
