6F7T

Crystal Structure of an Fab fragment in complex with a peptide from Bacillus subtilis RNase Y

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-03-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979340
Spacegroup name	P 21 21 2
Unit cell lengths	98.680, 130.450, 72.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.150 - 2.600
R-factor	0.204
Rwork	0.202
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qhr
RMSD bond length	0.010
RMSD bond angle	1.260
Data reduction software	XDS (0.7.14)
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.600	2.600
Number of reflections	29393
<I/σ(I)>	14.6	1.41
Completeness [%]	99.7	98.2
Redundancy	8.7	8.63
CC(1/2)	0.998	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.2M MgCl2; 0.1M TRIS; 16% (w/v) PEG 4000