6F7S

Crystal structure of Human ARS2 residues 147-270 + 408-763 with deletion of loop B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.966
Spacegroup name	C 2 2 21
Unit cell lengths	85.510, 148.270, 235.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	117.830 - 3.370
R-factor	0.25963
Rwork	0.258
R-free	0.30296
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f7j
RMSD bond length	0.007
RMSD bond angle	0.943
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	117.830	3.460
High resolution limit [Å]	3.370	3.370
Number of reflections	20293
<I/σ(I)>	12.62	1.97
Completeness [%]	94.3	67.1
Redundancy	4.12	3.82
CC(1/2)	0.998	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	281	Crystals of human ARS2 were obtained at 4 C in 2 microliter hanging drops with a 1:1 ratio of protein solution (6 mg per ml in 20 mM HEPES, 300 mM NaCl, 2 mM tris(2-carboxyethyl)phosphine, pH 7.8) to crystallisation solution. The crystallisation solution was 0.2 M potassium citrate tribasic monohydrate, 20 % (w/v) PEG 3350.