6F70
Crystal structure of glutathione transferase Omega 6S from Trametes versicolor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-05 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.060, 78.500, 67.860 |
Unit cell angles | 90.00, 106.56, 90.00 |
Refinement procedure
Resolution | 42.684 - 1.480 |
R-factor | 0.1577 |
Rwork | 0.157 |
R-free | 0.17950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f43 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.205 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.690 | 1.520 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.039 | |
Number of reflections | 85232 | |
<I/σ(I)> | 14.5 | |
Completeness [%] | 96.0 | |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 25 % PEG 1500, 0.1 M MMT pH 6.5 buffer (containing DL-malic acid, MES and Tris base in the molar ratios 1:2:2, respectively) |