6F64

Crystal structure of the SYCP1 C-terminal back-to-back assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	I 41 2 2
Unit cell lengths	43.380, 43.380, 292.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.627 - 2.493
R-factor	0.2271
Rwork	0.225
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.575
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	Arcimboldo
Refinement software	PHENIX ((1.12rc1_2807: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.910	2.580
High resolution limit [Å]	2.480	2.480
Rmerge	0.080	2.567
Rmeas	0.084	2.671
Rpim	0.023	0.727
Number of reflections	5424	591
<I/σ(I)>	14.8	1.5
Completeness [%]	99.8	99.7
Redundancy	13.2	13.2
CC(1/2)	1.000	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M sodium cacodylate pH 6.5, 1.4M sodium acetate; soaked in 0.1 M sodium cacodylate pH 6.5, 1.4 M sodium acetate, 20% PEG400