6F5X

Crystal structure of the SYCP1 N-terminal head-to-head assembly in closed conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.7712
Spacegroup name	I 2 2 2
Unit cell lengths	28.640, 39.380, 165.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.440 - 1.910
R-factor	0.2278
Rwork	0.227
R-free	0.23920
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.022
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.440	1.950
High resolution limit [Å]	1.910	1.910
Rmerge	0.028	0.678
Rmeas	0.036	0.873
Rpim	0.017	0.541
Number of reflections	7678	464
<I/σ(I)>	27.9	1.8
Completeness [%]	99.3	92.1
Redundancy	5.9	3.7
CC(1/2)	1.000	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	140 mM NaCl, 70 mM Na/K phosphate pH 6.2, 35% (v/v) PEG200; soaked in 40% (v/v) PEG200 and 100 mM NaI