6F5B

Structure of ARTD2/PARP2 WGR domain bound to double stranded DNA with 5'phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	63.160, 84.360, 95.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.21127
Rwork	0.208
R-free	0.27392
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PARP2 homology model
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.930
High resolution limit [Å]	2.800	2.800
Rmerge	0.010	1.797
Rmeas	0.021
Number of reflections	13124
<I/σ(I)>	11.36
Completeness [%]	99.8
Redundancy	4.77
CC(1/2)	1.000	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	12 % v/v Polypropylene glycol 400 0.1 M Na-acetate 3 % 2 propanol