6F4V
Crystal structure of cleaved Kallistatin complexed with heparin at 1.8 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-01 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 1.1960 |
| Spacegroup name | P 61 |
| Unit cell lengths | 113.770, 113.770, 76.561 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 98.530 - 1.800 |
| R-factor | 0.17343 |
| Rwork | 0.172 |
| R-free | 0.19636 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.458 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.880 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | |
| Number of reflections | 52290 | 3094 |
| <I/σ(I)> | 15.3 | 1.5 |
| Completeness [%] | 99.5 | 97.9 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% TERTBUTABOL |
