6F4H

Crystal structure of the Drosophila melanogaster SNF/U1-SL2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-18
Detector	PSI PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	C 2 2 21
Unit cell lengths	56.705, 150.028, 133.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.302 - 2.000
R-factor	0.1716
Rwork	0.170
R-free	0.21160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.259
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.000	2.000
Number of reflections	39021
<I/σ(I)>	15.8
Completeness [%]	99.7
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		277	0.2 M tri-lithium citrate, 20 % (w/v) PEG 3350 and 15 mM NiCl2 as an additive