6F4H
Crystal structure of the Drosophila melanogaster SNF/U1-SL2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-18 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 56.705, 150.028, 133.639 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.302 - 2.000 |
R-factor | 0.1716 |
Rwork | 0.170 |
R-free | 0.21160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.259 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 39021 | |
<I/σ(I)> | 15.8 | |
Completeness [%] | 99.7 | |
Redundancy | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 277 | 0.2 M tri-lithium citrate, 20 % (w/v) PEG 3350 and 15 mM NiCl2 as an additive |