6F3N
Crystal structure of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa cocrystallized with SAH in the presence of K+ and Zn2+ cations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.8950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.585, 99.275, 111.721 |
| Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
| Resolution | 24.820 - 1.850 |
| R-factor | 0.15136 |
| Rwork | 0.151 |
| R-free | 0.17648 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.599 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.082 | |
| Number of reflections | 152489 | |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 98.3 | |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 50 mM KH2PO4, 20% (w/v) PEG8000, 1 mM S-adenosyl-L-homocysteine |
