6F3L
The crystal structure of Glycogen Phosphorylase in complex with 10b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2017-10-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.646, 128.646, 116.276 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 129.000 - 1.900 |
| R-factor | 0.13829 |
| Rwork | 0.137 |
| R-free | 0.16146 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.311 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 129.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.163 | |
| Number of reflections | 76655 | |
| <I/σ(I)> | 10.8 | 3.4 |
| Completeness [%] | 99.5 | 98.9 |
| Redundancy | 14.9 | 15.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10 mM BES buffer |
