6F3J
The crystal structure of Glycogen Phosphorylase in complex with 10a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2017-10-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.539, 128.539, 116.455 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.890 - 2.200 |
| R-factor | 0.13777 |
| Rwork | 0.136 |
| R-free | 0.16686 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | native T-state of GPb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.264 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.000 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.229 | |
| Number of reflections | 49885 | |
| <I/σ(I)> | 10.3 | 5.5 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 10.6 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10 mM BES buffer |
