6F3J
The crystal structure of Glycogen Phosphorylase in complex with 10a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2017-10-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.539, 128.539, 116.455 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.890 - 2.200 |
R-factor | 0.13777 |
Rwork | 0.136 |
R-free | 0.16686 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | native T-state of GPb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.264 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 128.000 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.229 | |
Number of reflections | 49885 | |
<I/σ(I)> | 10.3 | 5.5 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 10.6 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10 mM BES buffer |