6F37

Fusion protein of RSL and trimeric coiled coil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980
Spacegroup name	P 21 3
Unit cell lengths	88.760, 88.760, 88.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.810 - 2.200
R-factor	0.2096
Rwork	0.208
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2BT9 chain A
RMSD bond length	0.006
RMSD bond angle	1.013
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	88.760	88.760	2.270
High resolution limit [Å]	2.200	9.070	2.200
Rmerge	0.178	0.065	0.511
Rmeas	0.185	0.069	0.531
Rpim	0.051	0.023	0.141
Number of reflections	12123	204	1019
<I/σ(I)>	21.6
Completeness [%]	100.0	99.9	100
Redundancy	13.2	9	14
CC(1/2)	0.997	0.998	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293.15	20% PEG 8000, 0.1 M CHES pH 9.5