6F34
Crystal structure of a bacterial cationic amino acid transporter (CAT) homologue bound to Arginine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2017-09-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.037, 82.699, 118.782 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.926 - 3.130 |
| R-factor | 0.233 |
| Rwork | 0.231 |
| R-free | 0.26190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oqt |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.474 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12-2829-000) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 64.630 | 64.630 | 3.210 |
| High resolution limit [Å] | 3.130 | 14.000 | 3.130 |
| Rmerge | 0.173 | 0.036 | 1.452 |
| Rmeas | 0.188 | 0.042 | 1.581 |
| Rpim | 0.074 | 0.021 | 0.618 |
| Total number of observations | 89902 | ||
| Number of reflections | 13951 | 195 | 1032 |
| <I/σ(I)> | 5.5 | ||
| Completeness [%] | 99.9 | 99.5 | 99.8 |
| Redundancy | 6.4 | 5.1 | 6.3 |
| CC(1/2) | 0.998 | 0.998 | 0.679 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4 | 292 | 28-34 % PEG 400, 0.1 M sodium acetate pH 4.0 and 0.1 M potassium fluoride, containing 10 mM of the amino acid ligand. |
