6F2Z

Structure of a Fc mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-17
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	71.280, 80.710, 113.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.740 - 2.300
R-factor	0.22132
Rwork	0.219
R-free	0.26792
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.280	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.770
Rmeas	0.083	0.952
Rpim	0.033	0.468
Number of reflections	28263	2044
<I/σ(I)>	14	1.8
Completeness [%]	95.3	72.7
Redundancy	5.8	3.5
CC(1/2)	0.992	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	295	20% PEG3350