6F2W

Bacterial asc transporter crystal structure in open to in conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.979260
Spacegroup name	P 41 21 2
Unit cell lengths	85.640, 85.640, 321.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.960 - 3.400
R-factor	0.23218
Rwork	0.230
R-free	0.26789
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f2g
RMSD bond length	0.016
RMSD bond angle	2.047
Data reduction software	xia2
Data scaling software	autoPROC (1.0.5)
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.900	3.460
High resolution limit [Å]	3.400	3.400
Rmerge	0.097	1.160
Rmeas	0.105	1.247
Rpim	0.039	0.454
Number of reflections	17358	849
<I/σ(I)>	8.8	1.5
Completeness [%]	99.5	99.76
Redundancy	7.1	7.2
CC(1/2)	0.999	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	28%PEG400 0,1M 0,1M Ammonium acetate pH8