6F2M
Structure of the bacteriophage T5 distal tail protein pb9 co-crystallized with 10mM Tb-Xo4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.63137 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.400, 95.240, 71.584 |
| Unit cell angles | 90.00, 102.66, 90.00 |
Refinement procedure
| Resolution | 48.160 - 1.800 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.20300 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.160 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.088 | |
| Rpim | 0.036 | |
| Number of reflections | 83616 | |
| <I/σ(I)> | 13.11 | |
| Completeness [%] | 96.6 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 100mM HEPES pH 7.5 / 8% Ethylene glycol / 6% - 16% PEG8000. Tb-Xo4 was directly mixed with the protein solution at a final concentration of 10 mM prior to crystallization. |
