6F1L
Lysozyme crystallized in presence of 100 mM sodium phosphate at pH 4.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.109, 56.165, 73.329 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.776 - 1.300 |
| R-factor | 0.1453 |
| Rwork | 0.143 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e3u |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.914 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.780 | 19.780 | 1.320 |
| High resolution limit [Å] | 1.300 | 7.120 | 1.300 |
| Rmerge | 0.058 | 0.021 | 0.709 |
| Rmeas | 0.061 | 0.023 | 0.744 |
| Rpim | 0.018 | 0.007 | 0.222 |
| Total number of observations | 309025 | ||
| Number of reflections | 29571 | 230 | 1484 |
| <I/σ(I)> | 19.4 | ||
| Completeness [%] | 94.3 | 95.8 | 98.1 |
| Redundancy | 10.5 | 8.7 | 10.8 |
| CC(1/2) | 1.000 | 1.000 | 0.888 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.5 M Sodium Chloride, 0.1 M Sodium Phosphate. 0.1 M Sodium Acetate |
