6F17
Structure of Mb NMH H64V, V68A mutant resting state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.592, 47.259, 76.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.162 - 1.450 |
R-factor | 0.1866 |
Rwork | 0.184 |
R-free | 0.21680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a6k |
RMSD bond length | 0.010 |
RMSD bond angle | 0.994 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.200 | 1.470 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.043 | 0.571 |
Number of reflections | 25715 | |
<I/σ(I)> | 19.3 | 2.6 |
Completeness [%] | 98.9 | 96.4 |
Redundancy | 6.2 | 6.4 |
CC(1/2) | 1.000 | 0.903 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M SPG buffer at pH 6.8-7.2 containing 28-32%(w/v) PEG1500. |