6F15
GLIC mutant H127Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2016-05-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.210, 133.100, 161.770 |
| Unit cell angles | 90.00, 102.82, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.850 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.22930 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.860 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 88185 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 100 mM NaAcetate pH 4 400mM NaSCN 12-15% PEG4000 165% glycerol 3%DMSO |
