6F0Z
GLIC mutant D88N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 190 |
Detector technology | PIXEL |
Collection date | 2015-09-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 181.930, 133.600, 160.220 |
Unit cell angles | 90.00, 101.96, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.2178 |
Rwork | 0.217 |
R-free | 0.22570 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.650 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 128919 | |
<I/σ(I)> | 11.2 | |
Completeness [%] | 99.7 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 12% PEG4K, 15% glycerol, 100 mM Acetate de Sodium pH4, 400 mM Sodium isothiocyanate, 3% DMSO |