6F0N
GLIC mutant E82A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2015-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9194 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.220, 132.600, 159.030 |
| Unit cell angles | 90.00, 101.65, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.200 |
| R-factor | 0.2106 |
| Rwork | 0.209 |
| R-free | 0.23250 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.310 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 60439 | |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 12% PEG4K, 15% glycerol, 100 mM Acetate de Sodium pH4, 400 mM Sodium isothiocyanate, 3% DMSO |
